METHOD FOR DIFFERENTIATION OF STRAINS OF VIBRIO CHOLERAE SEROGROUP 0139 BY ALKYL SULPHATE ACTIVITY Russian patent published in 2013 - IPC C12Q1/04 C12N1/20 

Abstract RU 2473697 C2

FIELD: medicine.

SUBSTANCE: invention refers to medical microbiology and concerns differentiation of toxigenic and non-toxigenic strains of cholera vibrios serogroup 0139. The described method involves preparing a synthetic medium 100 ml by using weights of: sodium chloride 0.5 g, agar 2 g, bromthymol blue 0.002 g; then the weight ingredients are dissolved in 0.01 M tris HCl buffer 100 ml, pH 7.8 and boiled for 30 minutes; the prepared medium is added with a substrate in the form of 1% aqueous sodium dodecyl sulphate (SDS) to the final concentration of 0.1% in the medium; it is followed by loop inoculation of the prepared medium on the analysed culture and incubation for 24-48 hours; the results are considered by the presence of milky-white areas 2-10 mm surrounding the inoculations on the agar sectors; the presence of those makes the strain to be referred to as toxicogenic (ctx+ tcp-), while the absence of those shows that the strain is non-toxicogenic (ctx+ tcp-).

EFFECT: method is simple and visual and may be used for determining the epidemic relevance of the strains of Vibrio Cholerae 0138 serogroup.

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RU 2 473 697 C2

Authors

Duvanova Ol'Ga Viktorovna

Mishan'Kin Boris Nikolaevich

Vodop'Janov Sergej Olegovich

Pisanov Ruslan Vjacheslavovich

Dates

2013-01-27Published

2011-04-27Filed