FIELD: medicine.
SUBSTANCE: invention refers to medical microbiology and concerns differentiation of toxigenic and non-toxigenic strains of cholera vibrios serogroup 0139. The described method involves preparing a synthetic medium 100 ml by using weights of: sodium chloride 0.5 g, agar 2 g, bromthymol blue 0.002 g; then the weight ingredients are dissolved in 0.01 M tris HCl buffer 100 ml, pH 7.8 and boiled for 30 minutes; the prepared medium is added with a substrate in the form of 1% aqueous sodium dodecyl sulphate (SDS) to the final concentration of 0.1% in the medium; it is followed by loop inoculation of the prepared medium on the analysed culture and incubation for 24-48 hours; the results are considered by the presence of milky-white areas 2-10 mm surrounding the inoculations on the agar sectors; the presence of those makes the strain to be referred to as toxicogenic (ctx+ tcp-), while the absence of those shows that the strain is non-toxicogenic (ctx+ tcp-).
EFFECT: method is simple and visual and may be used for determining the epidemic relevance of the strains of Vibrio Cholerae 0138 serogroup.
3 tbl, 3 ex
