METHOD FOR THE IDENTIFICATION OF VIBRIO CHOLERAE 01 SEROGROUP EL TOR BIOVAR Russian patent published in 2023 - IPC C12Q1/02 

FIELD: biotechnology.

SUBSTANCE: essence of the invention is using ROSTOV-13 bacteriophage strain, which previously undergoes a two-stage filtration in a tangential field, initially using a cassette with a hole diameter of 0.45 μm, then a cassette with a diameter of 0.22 μm, after which the purified bacteriophage is applied on the two-layer surface of the Petri dish, while the top layer includes 4.5 ml of 0.7% Marten agar, pre-melted and cooled to 45°C, then 0.5 ml of a four-hour broth culture of the Vibrio cholerae 01 strain of the biovar E1 Tor serogroup is added to it, the contents of the tube are mixed and quickly poured onto the surface of the bowl with 1.5% agar, representing the bottom layer of the bowl, then the agar is evenly distributed over the surface, and after solidification, bacteriophage drops are applied, which, when drained, form paths, the inoculation is incubated for 16–18 hours at a temperature of 37°C, identification is carried out on the grown culture by the presence of a zone of phage lysis, which confirms the presence of Vibrio cholerae 01 of the serogroup E1 Tor biovar or their absence by assessing lytic activity, which is taken into account on a five-point scale of the sacral system.

EFFECT: invention makes it possible to more reliably and qualitatively identify Vibrio cholerae 01 of the serogroup E1 Tor biovar.

1 cl, 3 tbl