FIELD: virology, medicine, veterinary, molecular biology.
SUBSTANCE: claimed method includes two-step PCR to produce single strain fluorescent labeled DNA fragment; hybridization on microarray, containing differentive oligonucleotide kit; date registration and interpretation. Results are evaluated by comparison of obtained data with set of standard, unique for each species templates, wherein said templates are selected in such a manner that fluorescent signal of perfect duplex allows unambiguous identification of genus Orthopoxvirus viruses. Differentive oligonucleotide kit, biological microarray, and kit useful in carrying out the method also are disclosed.
EFFECT: accelerated analysis method of increased accuracy.
8 cl, 1 dwg, 1 tbl, 1 ex
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Authors
Dates
2006-02-20—Published
2001-11-26—Filed