DIFFERENTIVE OLIGONUCLEOTIDE KIT FOR SPECIES IDENTIFICATION OF GENUS ORTHOPOXVIRUS VIRUSES, BIOLOGICAL MICROARRAY, KIT AND METHOD FOR SPECIES IDENTIFICATION OF GENUS ORTHOPOXVIRUS VIRUSES Russian patent published in 2006 - IPC C12Q1/68 G01N33/569 C07H21/00 

Abstract RU 2270252 C2

FIELD: virology, medicine, veterinary, molecular biology.

SUBSTANCE: claimed method includes two-step PCR to produce single strain fluorescent labeled DNA fragment; hybridization on microarray, containing differentive oligonucleotide kit; date registration and interpretation. Results are evaluated by comparison of obtained data with set of standard, unique for each species templates, wherein said templates are selected in such a manner that fluorescent signal of perfect duplex allows unambiguous identification of genus Orthopoxvirus viruses. Differentive oligonucleotide kit, biological microarray, and kit useful in carrying out the method also are disclosed.

EFFECT: accelerated analysis method of increased accuracy.

8 cl, 1 dwg, 1 tbl, 1 ex

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RU 2 270 252 C2

Authors

Mirzabekov Andrej Dar'Evich

Sandakhchiev Lev Stepanovich

Mikhajlovich Vladimir Mikhajlovich

Lapa Sergej Anatol'Evich

Mikheev Maksim Vjacheslavovich

Shchelkunov Sergej Nikolaevich

Dates

2006-02-20Published

2001-11-26Filed