FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes a nutrient medium containing components chosen in the following ratio, g/1 l: casaminic acids, 10-12; yeast extract, 3-5; potassium hydrogen phosphate, 7-8; magnesium sulfate, 0.1-0.2; sodium citrate dihydrate, 0.5-0.6; ammonium sulfate, 2-2.5, and glucose, 1-2. Using a liquid nutrient medium provides the intensive growth of staphylococci since first hours of cultivation and allows attainment of maximal yield of antibacterial compound after 8 h growth of culture. Invention is designated for culturing staphylococci in aim for preparing antibacterial peptide compounds.
EFFECT: improved and valuable properties of nutrient medium.
1 dwg, 2 tbl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
STAPHYLOCOCCUS HOMINIS KLP-1 STRAIN PRODUCING LOW-MOLECULAR PEPTIDE COMPOUNDS INHIBITING GRAM-POSITIVE BACTERIA DEVELOPMENT | 2010 |
|
RU2428470C1 |
STRAIN STAPHYLOCOCCUS WARNERI IEGM KL-1 AS PRODUCER OF LOW-MOLECULAR PEPTIDE COMPOUND INHIBITING CELLS GROWTH OF GRAM-POSITIVE MICROORGANISMS | 2001 |
|
RU2200195C2 |
STRAIN ENTEROCOCCUS MUNDTII PRODUCING PEPTIDE SUBSTANCE WITH ANTILISTERIAL ATIVITY | 2013 |
|
RU2532227C1 |
STREPTOMYCES sp. KMM 9044 - PRODUCER OF COMPOUNDS WITH ANTIBACTERIAL ACTIVITY | 2022 |
|
RU2784815C1 |
METHOD OF CEPHALOSPORIN C BIOSYNTHESIS WITH USING NEW ACREMONIUM CHRYSOGENUM STRAIN, RNCM NO F-4081D | 2009 |
|
RU2426793C2 |
METHOD FOR OBTAINING RECOMBINANT PROTEIN SAV-RGD, SPECIFICALLY RECOGNISING MELANOMA CELLS | 2013 |
|
RU2563540C2 |
METHOD OF PRODUCING OF RECOMBINANT PROTEIN SAV-RGD | 2014 |
|
RU2577138C1 |
SUPPRESSOR OF STAPHYLOCOCCI ENTEROTOXINE PRODUCING ACTIVITY | 2006 |
|
RU2325168C2 |
BACTERIOLYTIC COMPLEX, METHOD OF ITS PREPARING AND STRAIN FOR METHOD REALIZATION | 2000 |
|
RU2193063C2 |
FLUID NUTRIENT MEDIUM FOR HIGH-ACTIVITY METABOLITE COLLECTION BY BACILLUS PUMILUS 'PASHKOV' STRAIN SHOWING INHIBITORY ACTION ON BACTERIA, FUNGI AND VIRUSES | 2010 |
|
RU2460773C2 |
Authors
Dates
2006-04-20—Published
2004-05-05—Filed