METHOD FOR ASSESSMENT OF PREDISPOSITION TO ONCOLOGICAL DISEASES IN UROGENITAL SYSTEM Russian patent published in 2006 - IPC C12Q1/68 

FIELD: medicine, oncology.

SUBSTANCE: method involves study of gene GP3a and predisposition to oncological diseases being among them to invasive and metastatic forms is established in detection alleles PL-A1 and PL-A2 in this gene. Study of gene GP3a is carried out by isolation of DNA by the polymerase chain reaction method and this gene is analyzed in blood dry drop on a paper carrier. As primers for polymerase chain reaction the method involves using nucleotide sequences 5'-GCTCCAATGTACGGGGTAAA and 5'-CTCCTCAGACCTCCACCTTG, and DNA product with length 384 nucleotide pairs is synthesized that is cleaved then with restriction endonuclease Msp I to form DNA fragments, and allele PL-A1 is identified by fragment with length 290 and 80 nucleotide pairs, and allele PL-A2 - by fragments with length 175, 120 and 80 nucleotide pairs. Method provides rapid and with great reliability and safety to determine predisposition to oncological diseases in urogenital system.

EFFECT: improved assessment method.

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