FIELD: medicine.
SUBSTANCE: method involves comminuting and treating tissues with enzymes using collagenase solution in Eagle solution in Dulbecco modification, clearing from erythrocytes by means of lysing solution and filtering the obtained suspension next to it. Thymus is taken as human tissue. Collagenase of type II is applied for treating tissue with enzymes. Filtration is carried out by means of filters having pore size of 100 and 10 mcm.
EFFECT: high degree of cell mass uniformity.
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Authors
Dates
2006-07-27—Published
2004-04-09—Filed