METHOD FOR ISOLATION OF MESENCHYMAL STEM CELLS Russian patent published in 2005 - IPC

Abstract RU 2252252 C1

FIELD: biotechnology, in particular isolation of mesenchymal stem cells from human tissues for treatment of various diseases.

SUBSTANCE: human mesenchymal stem cells are isolated from fat tissue, decidual placenta membrane, placenta caul, or placenta chorial stroma. Human tissue is crushed and treated with collagenase solution in DMEM (Dulbecco's Modified Eagle Medium) wherein fat tissue, decidual placenta membrane or placenta caul is treated with type I collagenase, and placenta chorial stroma is treated with type IV collagenase. Obtained suspension is purified from erythrocytes with lysing reagent followed by filtration through filters with pore size of 100 mum and 10 mum.

EFFECT: increased cell suspension homogeneity, target product yield, and cell viability.

2 cl, 4 tbl, 4 ex

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RU 2 252 252 C1

Authors

Tepljashin A.S.

Dates

2005-05-20—Published

2004-04-09—Filed