FIELD: biotechnology, in particular cell therapy.
SUBSTANCE: invention relates to production of mesenchymal stem cells from human marrow. Uniform population of mesenchymal stem cells characterized with cell markers CD 44+, CD 45+, CD 34-, CD 90+, CD 105+, CD 106+ is isolated. Uniform cells are obtained on media interface during centrifugation of heparinized marrow and cells are subcultured in medium with addition cow foetus serum to produce cell biomass.
EFFECT: accelerated and inexpensive method for production of mesenchymal stem cells.
7 cl, 2 dwg, 2 tbl, 2 ex
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