FIELD: biotechnology and ecology.
SUBSTANCE: method comprises selecting and picking bioassay samples, objects to be tested and reference objects, adding bioassay to both objects and quantitatively evaluating effects of individual compounds or mixtures of different compounds on bioassays. More particularly, objects to be tested and reference objects of solid materials are prepared in the form of equilibrium solutions in water or another suitable aqueous solvent and bioassay is selected from one party of original species of living organisms, which are adapted to experiment conditions and kept simultaneously under the same conditions in test and reference objects (equilibrium solutions) during specified period of time. Thereafter, most sensitive parameter (response) of bioassay to the effect of contaminating substances on survived specimens in bioassay in test object is measured. From results of measurements of bioassay parameters using dependence of relative response of bioassay on concentration of dissolved components in test object, coefficient of biological activity of individual compounds toward bioassay, biological effect and biological activity of dissolved components in each sample are determined. From thus found results concerning biological activity of solutions and from relation of the mass of solid material and volume of solvent taken to prepare solutions, biological activity of solid materials is calculated.
EFFECT: enabled determination of toxic properties of water-soluble compounds in solid materials and values of negative effects thereof on living organisms.
4 cl, 5 dwg, 6 ex
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Authors
Dates
2006-08-10—Published
2005-08-22—Filed