METHOD OF DETERMINING BIOACTIVITY OF SOIL, WATER, WASTES AND PREPARATIONS BASED ON HUMIC SUBSTANCES Russian patent published in 2012 - IPC G01N33/18 G01N33/24 

FIELD: chemistry.

SUBSTANCE: disclosed is a method of determining bioactivity of soil, water, wastes and preparations based on humic substances. To this end, seeds are pre-soaked for a day in a solution of the analysed substance. The seeds are then let to germinate at 25°C for 3 days on a substrate mounted on a vertical transparent plastic container. The container is a base and a rectangular cover of equal size, which are placed in parallel and joined to each other on the entire perimeter by "button" type fasteners. The container allows for installation of a removable central horizontal partition wall for holding the substrate. Before germination, the substrate is moistened, a filter is placed on the substrate and seeds are placed on said filter in a single row along the line of the central horizontal partition wall at a distance of 1 cm from it. After germination, the root length of the sprouts is determined relative control. If the root length in the test samples is shorter than that of control samples by 20% or more, the analysed substance has a toxic effect, if the root length exceeds the control length, the analysed substance has a stimulating effect, in the rest of the cases, the analysed substance has no bioactivity or is weakly toxic.

EFFECT: method enables to conduct serial investigations, is characterised by simplicity and rapidness, seeds are exposed in a short period of time and there is no mechanical damage when determining root length.

3 dwg, 1 tbl, 1 ex