METHOD FOR DETERMINING DIRECT ECOLOGICAL DAMAGE CAUSED BY LIVING ORGANISM DEATH OUTCOME DUE TO ENVIRONMENT POLLUTION, USING NON-SELECTIVE BIOTESTS Russian patent published in 2007 - IPC G01N33/02 

FIELD: medicine.

SUBSTANCE: method involves selecting biotest objects from each living organism species party i, included into declared list, adapting the biotest objects to experiment conditions, placing the biotest objects simultaneously into test and control objects, or introducing aqueous solutions of materials under test into biotest organisms using known methods, holding the biotest organisms simultaneously at identical conditions in the test and control objects during given time in each applied biotest J, measuring the most sensitive biotest parameter (response) to action of components in test and control object. Exponential dependence of biological effect B_e on concentration C is proved. Survival parameters of living organisms in solutions are determined like biological effect B_e,i,j (relative response in tested solution series to control), biological activity coefficient of individual compound K_ij and biological activity of components B_ay. Then, biological effect value is determined relative to component action in tested medium and biological effects of equilibrium aqueous solutions under test, tested soil samples, bottom deposits and atmospheric air B_a,i,j,s, B_e,i,j,s and then, biological activity of gas medium or solid material is calculated with relations binding solvent volume Vs and gas medium volume under test V_gas or solid material mass m_hard taken in producing equilibrium solutions. Biological effect Pollutant action effect in gas medium or solid material upon biotest objects is calculated. Lost animals percent is determined from difference (100-B_e,i,j), (100-B_e,i,j,s), or (100-B_e,i,j,s)_hard in each object. The number of lost animals for each living organism species is determined in each biotest on the basis of known number of each living organism species in list or cadastre built in advance. The number of animals in each living organism species falling dead from environment pollution is determined as direct ecological damage.

EFFECT: high reliability of measurements.

3 cl, 2 dwg