FIELD: biotechnology, biochemistry, enzymes.
SUBSTANCE: invention reports about preparing a polypeptide possessing with the biological activity of enzyme protein-histidine-phosphatase and a polynucleotide encoding this enzyme also. Based on the polypeptide a pharmaceutical preparation is prepared using in pathological states associated with disturbance function of protein-histidine-phosphatase. Also, antibodies showing specificity to sites of indicated polypeptide are prepared. The use of the invention provides study of the N-phosphorylation process, carrying out the diagnosis of pathology of cellular regulation and growth cells and to regulate pathological states associated with disturbance in function of protein-histidine-phosphatase. Invention can be used in medicine and pharmacy.
EFFECT: valuable medicinal properties of enzyme, improved preparing method.
11 cl, 3 tbl, 10 dwg
