GLYCOSAMINOGLYCANS, DERIVATIVES OF K5 POLYSACCHARIDE, POSSESSING HIGH ANTI-COAGULANT AND ANTI-THROMBOSIS ACTIVITY AND METHOD FOR THEIR PREPARING Russian patent published in 2006 - IPC C08B37/00 C08B37/10 C12P19/26 A61K31/715 A61P7/00 

FIELD: bioorganic chemistry, chemical technology.

SUBSTANCE: invention describes N-deacylated N-sulfatized derivative of polysaccharide K5 epimerized up to at least to 40% of the content of L-iduronic acid with respect to the total content of uronic acids and with molecular mass 2000-30000 Da and comprising 25-50% by the chain weight showing the high affinity to ATIII and possessing anti-coagulant and anti-thrombosis activity with the ratio of abovementioned activities of HCII/antiXa from 1.5 to 4. Also, invention describes a method for preparing a derivative of polysaccharide K5 comprising isolation of polysaccharide K5 from Escherichia coli cells, N-deacylation and N-sulfatization, C-5-epimerization of D-glucuronic acid to L-induronic acid, supersulfatization, selective O-desulfatization, selective 6-O-sulfatization and N-sulfatization. The reaction of C-5-epimerization is carried out by using the enzyme glucuronosyl-C-5 epimerase in the soluble or immobilized form in the presence of bivalent cations chosen from the group comprising Ba, Ca, Mg and Mn.

EFFECT: improved preparing method, valuable medicinal properties of substances.

12 cl, 3 tbl, 12 dwg, 12 ex