N,O-SULFATIZED HEPAROSANS, METHOD FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION, POSSESSING ANTITHROMBOTIC ACTIVITY Russian patent published in 1997 - IPC

FIELD: medicine. SUBSTANCE: desired sulfatized N,O-hepatosans comprises chains or their mixture having molecular weight 1500-15000 has repeated disaccharide structure I given in specification. In said formula E is acetyl group, its quantity being 0-60 % of disaccharide units of N,O-sulfatized hepatosans, the rest disaccharide units comprise sulfate group and if it is necessary, hydrogen atom; G is hydrogen or sulfate group; degree of sulfatization of hepatosans (being expressed as ratio of sulfate and carboxyl) is 1.9-3. Compound having formula I is prepared by consequent steps, namely: a) cultivation of Escherichia coli strain; b) isolation and purification of N-acetylheparosan to prepare composition comprising 70-100 % of heparosan; c) partially deacetylation thus prepared composition; d) partially or complete N,O-sulfatization thus prepared composition. EFFECT: improved quality of desired product. 29 cl, 18 tbl