FIELD: biotechnology.
SUBSTANCE: methods for producing sulphated polysaccharide and producing 3'-phosphoadenosine-5'-phosphosulfate (PAPS) are provided. The methods include the step of obtaining a transformant from the genus Corynebacterium containing at least a gene encoding ATP sulfurylase and a gene encoding APS kinase. A reaction is then carried out to produce PAPS in a reaction solution containing ATP or an ATP source, a sulphate ion source and a transformant or processed material thereof. Sulfation is carried out using a transformant containing a gene encoding sulfotransferase.
EFFECT: inventions make it possible to produce sulphated polysaccharides using bacterial cells and inexpensive raw materials such as magnesium sulphate, without enzyme purification or the addition of PAPS.
12 cl, 9 dwg, 1 tbl, 12 ex
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