INSULIN PRECURSOR, METHOD FOR PRODUCTION AND USE THEREOF Russian patent published in 2006 - IPC C07K14/62 C12N15/17 C12P21/00 

FIELD: biotechnology, in particular medical and biological industry.

SUBSTANCE: invention relates to new polypeptides namely precursors of insulin or analogs thereof, whish are characterized by increased structure stability and high expression levels during production thereof in recombinant forms in yeast cells. Polypeptides of present invention unlike natural form of insulin precursors contain shortened C-peptide including aromatic amino acid group and have general formula B (1-27)-X₂-X₃-X₁-Y-A (1-21), wherein X₁ is peptide sequence of 1-3 amino acid rests, wherein aromatic acid rest is in close vicinity with Y; X₂ represents Pro or Asp; X₁ represents Lys, Y-Lys, or Arg; A (1-21) is A-chain of human insulin; B(1-27) is the first 27 amino acid rests of human insulin D-chain rests.

EFFECT: method for production of new insulin precursors by recombinant DNA technology followed by conversion thereof in active hormone.

9 cl, 10 dwg, 6 tbl, 5 ex