FIELD: biotechnology, biochemistry, enzymes, microbiology.
SUBSTANCE: invention proposes a method for microbiological oxidation of N-, O- or S-heterocyclic mono- or multinuclear aromatic compounds. The method involves culturing the recombinant microorganism that expresses cytochrome P-450-dependent monooxygenase BM-3 from Bacillus megaterium with amino acid sequence represented in SEQ ID NO:2 that comprises at least one functional mutation in region 86-88 and, if necessary, at least one functional mutation in one of regions 73-82, 172-224, 39-43, 48-52, 67-170, 300-335 and 352-356. The prepared oxidation product is isolated from medium, Invention provides carrying out oxidation of organic compounds with enhanced degree of effectiveness.
EFFECT: enhanced effectiveness of enzymes.
17 cl, 1 tbl, 7 ex
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| MODIFIED CYTOCHROME P-450 OF MONOOXYGENASE (VARIANTS), METHOD OF OXIDATION OF COMPOUND TAKEN FROM GROUP CONTAINING POLYCYCLIC AROMATIC HYDROCARBON, LINEAR OR BRANCHED ALKANE, DIPHENYL OR BIPHENYL COMPOUND INCLUDING HALOGEN DERIVATIVES AND HYDROGEN HALIDES | 1995 |
|
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| CYTOCHROME P450-MONOOXYGENASES | 1998 |
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