FIELD: biotechnology; biochemistry.
SUBSTANCE: invention relates to the field of biotechnology and biochemistry, in particular to an isolated P450 enzyme. Said enzyme differs in that it consists of an amino acid sequence that is at least 80 % identical to SEQ ID NO: 1, which comprises threonine at position 225 and aspartic acid at position 289. Present invention relates to a host cell for producing said P450 enzyme and to a microorganism expressing this enzyme and intended for producing a steroid hormone precursor. Invention also relates to methods for producing a steroid hormone precursor.
EFFECT: invention enables to obtain a P450 enzyme with increased enzyme activity for the production of a steroid hormone precursor.
14 cl, 5 dwg, 4 ex
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