METHOD FOR SELECTIVE ASSAY OF NONYLPHENOL IN SOLUTION BY USING PIEZOELECTRIC CRYSTAL IMMUNOSENSOR Russian patent published in 2006 - IPC G01N33/53 C12Q1/00 C12R1/00 

FIELD: analytical chemistry, immunology.

SUBSTANCE: invention relates to a method for selective determination of nonylphenol that is carried out by using a piezoelectric crystal immunosensor. Sample containing nonylphenol and fixed amount of antibodies raised to its in phosphate buffer (pH = 7.1-7.5) is passed through a flow-type cell comprising mass-sensitive piezoelectric crystal immunosensor with receptor cover based on aminophenol-protein conjugate. Nonylphenol is determined by recording change of the sensor frequency oscillation in interaction of aminophenol-protein conjugate with antibodies to nonylphenol wherein the sensor frequency oscillation change is inversely with the nonylphenol concentration in a sample to be analyzed. Then method involves regeneration of receptor cover for carrying out the following assays by passing 0.02-0.10 mM solution of potassium thiocyanate through a cell. Using the invention provides carrying out multiple selective analysis with the detection limit of nonylphenol 0.8 ng/ml and to determine the concentration of nonylphenol in the range 1-20 ng/ml.

EFFECT: improved assay method.

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