METHOD OF STREPTOMYCIN DETECTION BY MEANS OF PIEZOELECTRIC IMMUNOSENSOR Russian patent published in 2011 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention describes a method of immunochemical streptomycin detection wherein on a surface of a piezoelectric immunosensor, a receptor coating with a streptomycin-protein (bovine serum albumin) conjugate is immobilised, a settled number of streptomycin antibodies is added to a sample and kept for 2-3 min to produce an immune complex which is introduced in a cell in a phosphate buffer solution of pH = 7.1-7.5 and to record the sensor oscillation frequency variations when reacting with the streptomycin-protein conjugate with streptomycin antibodies, an analytical signal is inversely proportional to the concentration of streptomycin in the analysed sample, while the concentration is derived from the calibration chart, the receptor coating is regenerated by applying 0.03 mM potassium thiocyanate solution on the surface.

EFFECT: higher sensitivity of streptomycin detection in composite mixtures, ensured multiple use of the immunosensor after regeneration of the bioreceptor coating that reduces analysis price.

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