METHODS FOR IDENTIFICATION, ISOLATION AND PRODUCTION OF ANTIGENS FOR SPECIFIC PATHOGEN Russian patent published in 2006 - IPC G01N33/53 A61K39/00 A61K39/395 A61P31/00 

FIELD: medicine, vaccines.

SUBSTANCE: disclosed is method for identification, isolation and production of antigens interacting with hyperimmune serum from specific pathogen useful in vaccines for human or animals. Claimed method includes providing of antibody preparation from serum pool of certain animals or human or individual serums with antibodies against certain pathogen; production of at least one expression library of certain pathogen; data screening of said at least one expression library using abovementioned antibody preparation; identification of antibodies binding to antibodies in antibody preparation during screening; screening of identified antigens using individual antibody preparations from individual serums obtained from subjects having antibodies against said certain pathogen; identification of reacting with hyperimmune serum antigen part of identified antigens than bind to relevant part of individual antibody preparations from individual serums optionally isolation of reacting with hyperimmune serum antigens and production thereof by chemical or recombinant methods.

EFFECT: improved method for identification of effective antigens for certain pathogen useful as preferable candidates of antigen vaccines.

20 cl, 11 dwg, 10 tbl, 7 ex