METHOD FOR DIRECT ELECTRON-MICROSCOPIC DIAGNOSTICS FOR DETECTING SEVERITY DEGREE OF BACTERIAL ENDOTOXICOSIS IN CASE OF PURULENT-NECROTIC PULMONARY DISEASES Russian patent published in 2007 - IPC G01N33/49 

FIELD: medicine, pulmonology.

SUBSTANCE: one should apply 0.1 ml plasma onto the edge surface of electron microscope's stage and after its drying it should be sprayed with carbon in vacuum equipment, inspect in scanning electron microscope, take into account blood plasma-visualized initial bacterial cells (IBC), unbalanced cell growth (UCG), filamentary bacterial structures (FBS), conglomerates (C), deformed spheroidal cells (DSC) and elementary bodies (EB) of bacterial L-forms and according to their availability, quantity, sizes and functional state one should diagnose the severity degree of bacterial endotoxicosis in case of active pulmonary tuberculosis and pulmonary abscess of staphylococcal nature. Application of the present innovation provides the chance to visualize circulating microscopic and submicroscopic bacterial forms at separate stages of endotoxicosis, the dynamics of their quantity and the site of development.

EFFECT: higher objectivity and accuracy of diagnostics.

2 cl, 2 dwg, 6 ex, 1 tbl