POLYPEPTIDE WITH BIOLOGICAL ACTIVITY OF INHIBITOR OF COLLAGEN-INDUCED ADHESION OF PLATELETS, ITS PREPARING AND USING Russian patent published in 2007 - IPC C07K14/435 C12N15/12 C07K16/18 A61K38/17 

FIELD: biochemistry, biotechnology, peptides.

SUBSTANCE: invention relates to protein of molecular mass 12000 Da isolated from medicinal leech saliva (Hirudo medicinalis) comprising 6 cysteine residues able to form bonds -S-S-, and pI value about 3.7. The novel protein possesses ability to block platelets adhesion induced by collagen. As result of screening cDNA library of H. medicinalis a novel gene of new inhibitor of adhesion is identified and its nucleotide sequence is determined. Invention describes a method for preparing recombinant form of protein involving transformation of suitable cells with vector comprising DNA sequence encoding inhibitor, culturing cells under condition providing its expression and isolation of expressed protein. Invention proposes using natural and recombinant forms of inhibitor in therapy states associated with vessels congestion and diseases of circulatory system, and for treatment of articles surface made of natural and artificial collagen.

EFFECT: valuable biological and medicinal properties of polypeptide.

17 cl, 10 dwg, 13 ex