FIELD: food industry.
SUBSTANCE: mixture of D-pantotenic acid and its salts is prepared via culturing of D-pantotenic acid-producing microorganism, wherein pantotenic acid (pan) and/or isoleucine/valine (ilv) biosynthesis control is spoiled and which, in the fermentation process in culturing medium, produces at least 2 g/L D-pantotenic acid salt, while no free β-alanine and/or β-alanine salt is added to the culturing medium. Fermentation solution containing D-pantotenate is then passed through one or several ion-selective membrane under action of electric field resulting in that low-molecular weight ions are removed from D-pantotenate-containing solution and wherein content of D-pantotenic acid salts in the form of monovalent cations is lowered to concentration below or equal to 1 g/kg. PH value of solution containing free D-pantotenic acid is adjusted to 3-10 by adding calcium and/or magnesium bases and thus obtained solution containing calcium and/or magnesium pantotenate is dried and formatted. Thus obtained formulation is useful as feed additive.
EFFECT: simplified process and reduced amount of undesired impurities.
23 cl, 1 tbl, 3 ex
Title | Year | Author | Number |
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Authors
Dates
2007-01-27—Published
2002-02-20—Filed