FIELD: biotechnology.
SUBSTANCE: disclosed is a method of producing riboflavin, comprising the steps of growing the bacterium Bacillus subtilis – a riboflavin producer in a fermentation medium containing sucrose, dry yeast, corn extract, monosubstituted potassium phosphate, disubstituted potassium phosphate, under aerobic conditions and extracting riboflavin from the culture fluid, soya flour in amount of 5-20 g/l and ammonium sulphate in amount of 5-10 g/l are added to the fermentation medium, while maintaining the pH value of the fermentation medium at 7.0±0.2.
EFFECT: invention provides high output of the end product.
1 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING RIBOFLAVIN, STRAINS BACILLUS SUBTILIS AS PRODUCERS OF RIBOFLAVIN (VARIANTS) | 2002 |
|
RU2261273C2 |
| STRAIN OF BACILLUS SUBTILIS - A PRODUCER OF RIBOFLAVIN (VARIANTS) | 1994 |
|
RU2081175C1 |
| STRAIN OF BACTERIUM BACILLUS SUBTILIS - A PRODUCER OF RIBOFLAVIN | 1994 |
|
RU2081906C1 |
| BACTERIA Bacillus subtilis PRODUCING 5`-AMINOIMIDAZOLE-4-CARBOXAMIDERIBOSIDE (AICAR), AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF AICAR BY CULTURING THIS BACTERIUM | 2013 |
|
RU2542387C1 |
| METHOD FOR MICROBIOLOGIAL SYNTHESIS OF PURINE NUCLEOSIDE OF 5'-AMINOIMIDAZOLE-4-CARBOXAMIDE RIBOSIDE (AICAR) AND BACILLUS SUBTILIS STRAIN - PRODUCER OF AICAR | 2008 |
|
RU2405833C2 |
| BACILLUS GENUS BACTERIA PRODUCING HYALURONIC ACID, AND METHOD OF PRODUCING HYALURONIC ACID USING SAID BACTERIA | 2019 |
|
RU2719140C1 |
| METHOD FOR PREPARING POLY-GAMMA-GLUTAMIC ACID AND MICROORGANISM USED IN THIS METHOD | 2005 |
|
RU2313578C2 |
| COMBINED PREPARATION BASED ON STRAINS PENICILLIUM CITRINUM BKM F-4930D, BASILLUS SUBTILIS BKM B-3171D AND BACILLUS LICHENIFORMIS BKM B-3172D, METHOD FOR PRODUCTION THEREOF | 2024 |
|
RU2834047C1 |
| METHOD OF OBTAINING RIBOFLAVIN | 0 |
|
SU908092A1 |
| METHOD OF OBTAINING L-THREONINE | 2006 |
|
RU2402610C2 |
