FIELD: veterinary immunology.
SUBSTANCE: the present innovation deals with detecting the level of antibody-formation in animal body. It is necessary to supplement the lymphocytes isolated from cattle's blood about 1.0-3.0%-citric acid solution at volumetric ratio of 5-10:1, wash with phosphate buffer at pH=7.2 to be incubated with 5-15% solution of equine serum in RPMI-1640 medium at 4°C, fixation of lymphocytes should be conducted before adding an antigen, moreover, as antigen one may apply monoclonal antibodies to cattle's immunoglobulin M. Monoclonal antibodies to cattle's immunoglobulin M should be applied at 1:10000-20000 dilution. The innovation provides the chance to detect cytoplasmatic and membranous immunoglobulins of animals' lymphocytes and, also, the level of antibody-formation in animals' body.
EFFECT: higher accuracy of detection.
1 cl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR MULTIPLEX ANALYSIS OF B CELLS FOR ASSESSING THE CELLULAR LINK OF THE IMMUNE SYSTEM OF CATTLE | 2020 |
|
RU2761468C1 |
| STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS, MUS MUSCULUS L | 0 |
|
SU1705343A1 |
| COMPOUND STIMULATING DURING EXPERIMENT SYNTHESIS OF IMMUNOGLOBULINS | 1995 |
|
RU2108096C1 |
| STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS MUS MUSCULUS L, PRODUCING MONOCLONAL ANTIBODIES FOR SURFACE PROTEIN OF CATTLE CORONAVIRUS | 0 |
|
SU1661231A1 |
| STRAIN 8C12 OF PERMANENT INTER-SPECIES HYBRID LINE OF CELLS OF MOUSE Mus. musculus AND SHEEP Ovis aries - PRODUCENT OF MONOCLONAL ANTIBODIES TO GLYCOPROTEIDAL ANTIGEN OF VIRUS OF CATTLE LEUCOSIS | 2008 |
|
RU2377297C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-10 - PRODUCER OF MONOCLONAL ANTIBODY 5H/E TO ANTIGEN KDA 200 OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570638C1 |
| STRAIN 5A10 OF PERMANENT HYBRIDOMAL LINE OF CELLS OF MOUSE Mus. musculus - PRODUCENT OF MONOCLONAL ANTIBODIES TO IgG OF CATTLE | 2008 |
|
RU2377299C1 |
| STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS, MUS MUSCULCS L. PRODUCING MONOCLONAL ANTIBODIES FOR NUCLEOCAPSIDE PROTEIN OR CATTLE VIRUS | 0 |
|
SU1666532A1 |
| MOUSE HYBRIDOMA 56 - PRODUCER OF MONOCLONAL ANTIBODY SHOWING SPECIFICITY TO CORE ANTIGEN OF HUMAN PROLIFERATING PCNA CELLS | 2010 |
|
RU2435850C1 |
| STRAIN 1H8 OF PERMANENT HYBRIDOMAL LINE OF CELLS OF MOUSE Mus. Musculus - PRODUCENT OF MONOCLONAL ANTIBODIES TO IgG OF SHEEP | 2008 |
|
RU2377298C1 |
