METHOD FOR OBTAINING ALPHA5-INTERFERON PROTEIN Russian patent published in 2016 - IPC C12P21/02 C12N1/38 C12N1/20 A61K38/21 

FIELD: chemistry.

SUBSTANCE: invention relates to field of biotechnology and can be used for recombinant production of α5-interferon. E. coli cell - IFNa5 producent is cultivated with application of carbon solution to feeding with glucose as carbon source, containing the following components per litre, mg: 90-111 of iron (III) chloride hexahydrate, 12.15-14.985 of calcium chloride dehydrate, 20.25-24.975 of zinc (II) sulphate heptahydrate, 4.5-5.55 of manganese (II) sulphate monohydrate, 9-11.1 of copper (II) sulphate pentahydrate, 3.42-4.218 of cobalt chloride hexahydrate, 0.9-1.11 of sodium molybdate dehydrate and 2.07-2.553 of boric acid. Fermentation medium is, in fact, free of animal- or yeast-derived components and is characterised by content of the following components per litre, mg: 28.5-36.9 of iron (III) chloride hexahydrate, 3.8475-4.9815 of calcium chloride dehydrate, 6.4125-8.3025 zinc (II) sulphate heptahydrate, 1.425-1.845 of manganese (II) sulphate monohydrate 2.85-3.69 of copper (II) sulphate pentahydrate, 1.083-1.4022 of cobalt (II) chloride hexahydrate, 0.285-0.369 of sodium molybdate dehydrate and 0.6555-0.8487 of boric acid.

EFFECT: invention makes it possible to minimise incorporation of additional methionine residue into N-terminal part of polypeptide chain and generation of its oxidised molecules.

10 cl, 13 dwg, 3 tbl, 4 ex