FIELD: chemistry.
SUBSTANCE: extraction is done using affinity chromatography with immobilised metal. The method can be realised in native conditions. Biologically active G-CSF is obtained with purity over 95%. Two more chromatography stages are done, cation-exchange and gel filtration, to remove trace amounts of impurities. The method allows for obtaining G-CSF with high output and over 99% purity.
EFFECT: described method is especially suitable for industrial production of G-CSF.
16 cl, 5 dwg, 5 ex
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