FIELD: medicine, in particular drug screening for tuberculosis treatment.
SUBSTANCE: diagnosis is carried out by determination of tuberculosis mycobacteria sensitivity to isoniaside based on mutation detection in katG, inhA, oxyR/ahpC genes by conformational polymorphism of single-strain fragments and detection mutation in katA gene, which is responsible for abovementioned preparation resistance additionally in 10 % experiences. Also disclosed are primers for DNA of tested katA gene, amplification conditions and electrophoresis conditions. Nucleotide sequences of primer pair are disclosed in specification. Buffer containing in 30 mul Tris-HCl 10 mM, pH 8.8; KCl 50 mM; 0.5 % of Twin 20, formamide 5 %; MgCl 2.0 mM MgCl2; Tag-polymerase 1.5 U; each mucleoside triphosphate 200 muM; each oligonucleotide primer 0.1-0.15 muM; and sample 3 mul is used for amplification.
EFFECT: improved method for diagnosis of mycobacterium tuberculosis strain sensitivity to isoniaside.
| Title | Year | Author | Number |
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