IMMUNOCHROMATOGRAPHY METHOD FOR DETECTION OF ESCHERICHIA COLI IN WATER Russian patent published in 2007 - IPC G01N33/558 C12Q1/04 C12R1/19 

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a novel method for immunochromatography detection of Escherichia coli in water using a test-band. Test-band consists of A, B and C assembled on backing D wherein part of A overlaps part B by 1-2 mm. Part A represents inert porous carrier made of fiber glass (PED) and comprises reaction zone 1 applied on it (with applied conjugate of rabbit monospecific antibodies raised to E. coli with horse radish peroxidase) and reaction zone 2 (with applied conjugate of murine monoclonal antibodies labeled with colloidal gold against horse radish peroxidase). Conjugates are applied on reaction zone by parallel bands in the central region of PED and perpendicularly to liquid flow in distance 2 mm between conjugates. Part B in test-band represents nitrocellulose immobilized on lavsan base with applied test-zone 6 and control zone 7. Test-zone 6 represents zone with applied band parallel to long side of test-band for 2 mm from middle of nitrocellulose with rabbit monospecific antibodies against E. coli. Control zone 7 represents zone with applied by parallel band for 3 mm from middle and nearer to suction filter C by rabbit monospecific antibodies against murine immunoglobulins. Part B shows a capillary linkage with reaction zone of carrier A. C-suction filter is used for absorption of unreacted reagents. Presence of Escherichia coli is estimated by the presence of color of test-zone 6. Color of control zone shows working capacity of test-band. Using invention provides universality of band in detection of any type of strains Escherichia coli with sensitivity 1-10^-8 g/ml.

EFFECT: improved method for detection.

3 dwg, 1 ex