FIELD: biotechnology, medicals.
SUBSTANCE: invention relates to method for extraction, purification, and enzyme modification of β-conglycinin α'-subunit. Claimed method includes isolation of β-conglycinin by selective extraction from milled defatted soybeans and following deposition by extract treatment with ethanol aqueous solution. Enriched fraction is then subjected to affinity chromatography with metal (MAC) under denaturant condition to produce α'-subunit which further is treated with chymotrypsin and subjected to additional treatment with MAC to isolate amino-terminated region of said polypeptide having molecular weight of 28000 Da.
EFFECT: isolated fraction of increased purity.
10 cl, 2 tbl, 2 dwg, 1 ex
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