FIELD: biotechnology, recombinant polypeptides. SUBSTANCE: method involves making hybrid DNA - a structure encoding the protein in which two polypeptide sequences are bound by transient region containing site recognized by Ig A-protease. The host cells are transformed with recombinant expression vector containing the indicated structure, isolation of the obtained fusion protein, its treatment with Ig A-protease at weight ratio of enzyme to substrate from 1: 1 to 100:1 at pH 6.5-8.5 and isolation of peptide. Specific forms of hybrid DNAs and fusion proteins are obtained, characterized and tested. Invention provides preparing methionineless polypeptides. EFFECT: improved method of synthesis, increased expression yield and purification degree of proteins. 19 cl, 11 ex, 4 dwg
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