METHOD FOR DETERMINATION OF PHENOTHIAZINE DERIVATIVES IN BIOLOGICAL FLUIDS Russian patent published in 2007 - IPC G01N33/48 

FIELD: analytical chemistry, clinical laboratory diagnosis.

SUBSTANCE: invention relates to methods used in determination of phenothiazine derivatives in biological objects by thin layer chromatography method. Method involves treatment of analyzed biological fluid sample with alkali, extraction of analyzed substances, dosing extract in inert gas flow and chromatography separation of phenothiazine derivatives. Treatment of biological fluid sample is carried out with ethanol and alkali taken in the ratio = 10:1:1, respectively, to pH value 10-13. Extraction of analyzed substances is carried out on solid sorbent using supercross-linked polystyrene, extract is washed out from sorbent with chloroform taken in the amount 8 ml, not above and dried again in nitrogen flow. Chromatography separation of phenothiazine derivatives is carried out by thin layer chromatography method wherein a mixture acetone and ammonia taken in the ratio = 100:1, respectively, is used. Invention provides simplicity, availability, effectiveness and high selectivity of method.

EFFECT: improved assay method.

2 cl, 4 dwg, 2 ex