FIELD: agriculture, plant tissue cultivation.
SUBSTANCE: seeds of monocotyledon plant are treated under vacuum infiltration conditions for 30-50 min in suspension of Agrobacterium tumefaciens GV3850 strain and further incubated in suspension of this strain for 23-25 hours. Germinants are grown on solid nutrient medium for 3-5 days without antibiotics, then kanamycin and cefotaxime are added together.
EFFECT: increased yield of transgenic monocotyledon plant regardless of morphogenetic potential and somaclonal variability thereof; accelerated method for producing such plants.
2 dwg, 1 tbl, 54 ex
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