FIELD: gene and protein- engineering, medicine, pharmaceutical industry.
SUBSTANCE: invention relates to recombinant plasmide DNA pER-Gl is constructed, which provides synthesis of hybrid polypeptide containing human glucagone and intein in Escerichia coli cells. Producer of said hybrid polypeptide is obtained by transformation of E.coli ER2566 with pER-Gl plasmide. Method for production of human recombinant glucagone includes providing and cultivation of hybrid polypeptide producer containing human glucagone and intein followed by isolation and cleavage of said hybrid protein.
EFFECT: increased yield of human recombinant glucagone; simplified technology.
3 cl, 3 ex, 3 dwg
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Year |
Author |
Number |
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