FIELD: chemistry.
SUBSTANCE: invention refers to biochemistry, namely to recombinant plasmid DNA pER-ARNS3 providing the synthesis of a hybrid polypeptide comprising ARNS3 and mini-intein Ssp DnaB, in Escherichia coli cells. The said plasmid DNA comprises BsaBI/Eco0109I-DNA plasmid fragment pTWIN-1 gene translation amplifier 10 of phage T7, NdeI/BamHI-DNA fragment containing the mini-intein gene Ssp DnaB and sequence of ARNS3 recombinant gene adapted to these sites. The plasmid also contains a b-lactamase gene that determines the resistance of E. coli cells transformed by plasmid pER-ARNS3 to penicillin antibiotics, as a genetic marker and unique restriction endonuclease recognition sites. This invention also discloses a strain of Escherichia coli C3030/pER-ARNS3, producing the said fusion polypeptide. This invention also discloses a method for production of the said ARNS3 recombinant protein, comprising culturing of the said strain of Escherichia coli C3030/pER-ARNS3.
EFFECT: invention provides recombinant ARNS3 with high yield according to a simplified technology.
3 cl, 4 dwg, 4 ex
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