FIELD: production methods.
SUBSTANCE: cells Escherichia coli, transform the plasm DNA pER-hEGF with producing of recombination stamp Escherichia coli ER2566/pER-hEGF - producer of hybrid protein Int-hEGF, includes with the structure of recombinated epidermal factor of personal growth the structure of inteine. It is provided the cultivation of achieved stamp Escherichia coli ER2566/pER-hEGF with following cell distraction in buffer substance by ultrasound, after the cell distraction hybrid protein is separated as the body of inclusions, they are dissolved in buffer, contains 6 M carbamide, renatured the hybrid protein, adsorbed on the chitinous sorbent, and then is it raised auto-catalyc splitting with рН changing from 10.0 to 7.0 with generating the solid product, which is eluted from sorbent.
EFFECT: invention allowes to obtain high productive recombinated stamp of bacterial stamp-producer of hybrid protein.
3 cl, 3 dwg, 5 ex
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