METHOD OF ACCELERATED ACQUIRING OF MESENCHYMAL STEM CELLS (MSC) CULTURE OF MAMMALS, EXCEPT HUMAN, WITH LOW HETEROGENEITY AND HIGH VIABILITY Russian patent published in 2008 - IPC C12N5/06 

FIELD: medicine.

SUBSTANCE: invention belongs to the area of biotechnology and medicine. Precursor cells are recovered and then cultivated till obtaining of desired cell culture. Growth of cultured cells takes from 4 to 10 days in hypoxic condition, with oxygen content no less than 5%, the cells from 1^st to 2^nd passages are being used. Quantity of apoptosis and necrotic damaged cells and their morphological characteristics are determined. According to markers CD90, CD54, CD44, CD73, CD11b, and CD45 expression, immune type of cells is determined. If aggregate quantity of apoptosis and necrotic damaged cells is reduced two times and more compared to representative cultures in normoxia conditions, if MSC immune type is retained completely, and if quantity of rapidly dividing uniform cells exceeds quantity of slow proliferating large cells, then received culture is reputed as a culture of mesenchymal cells with low heterogeneity and high viability.

EFFECT: increase of proliferative activity of stromal precursor cells culture, reduction of heterogeneity of culture.

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