FIELD: immunobiotechnology.
SUBSTANCE: method for isolating antibodies involves a procedure of adsorption chromatography using water-insoluble uncharged adsorbent (variant 1), or a procedure for desalting antibodies from egg yolk aqueous extract resulting to specific precipitation of IgY antibodies (variant 2), or using a successive procedure of adsorption chromatography (by variant 1) and desalting procedure (variant 2) in combination (variant 3). Invention provides carrying out the selective purification of antibodies IgY (▵Fc) and IgY from lipids under conditions of their increased content in egg yolk of Anseres order birds. Invention can be used for quantitative and qualitative analysis or preparing pharmaceutical compositions directed to etiological factor representing the interest.
EFFECT: improved preparing method.
23 cl, 4 dwg, 2 tbl, 6 ex
Authors
Dates
2007-08-20—Published
2002-06-10—Filed