METHOD FOR COMPLEX REPROCESSING OF CAKE URCHINS Russian patent published in 2007 - IPC A61K35/56 

FIELD: production of biologically active substances.

SUBSTANCE: claimed method includes raw material treatment with aqueous solution of organic complexion agent; extract concentration to produce protein concentrate. Then alkali aqueous solution is added to raw materials; alkali extraction is carried out; alkali extract is concentrated to produce concentrate I. Further 75-80 alcohol solution of organic complexion agent is added to raw materials and mixture is infused for 20 hours. Extract is concentrated to produce concentrate II. Further 10 % alcohol solution of 30 % sulfuric acid is added to raw materials, mixture is infused and concentrated to produce concentrate III. Then sulfuric acid is added solution is added to raw materials again, mixture is infused and concentrated to produce concentrate IV. Diluted sulfuric acid solution is added again, mixture is infused and concentrated to produce concentrate V. Concentrates I, II, III are separately purified wherein equal hexane volume is added to each concentrate, mixture is agitated and conditioned up to phase separation. For concentrates I and II such procedure is repeated three times. Bottom aqueous-alcohol phase is separated, concentrated up to dry state and cold acetone is added. Mixture is intensively shacked to produce acetone extract with insoluble in acetone deposit. Such procedure is doubly repeated and after acetone removing rest with ganglioside mixture is stored in ethanol at -4°. Equinochrome A is obtained from concentrate III by addition of equal hexane volume, mixture agitating and conditioning up to phase separation. Bottom aqueous-alcohol phase is separated, twice purified with hexane. Further chloroform fraction with equinochrome A is recovered in aqueous-alcohol phase/chloroform ratio of 1:2. Such procedure is repeated three times. Obtained chloroform concentrates are combined, concentrated, purified from impurities with bivalent metal salt aqueous solution in chloroform extract ratio of 2:1, and concentrated up to dry state.

EFFECT: enhanced spectrum of biologically active substances.

2 cl, 4 ex