PLASMID VECTOR pHYP WITH HIGH SEGREGATION STABILITY FOR EXPRESSION OF RECOMBINANT PROTEIN, BACTERIUM - PRODUCENT OF PRECURSOR OF RECOMBINANT PROTEIN AND METHOD TO PRODUCE RECOMBINANT PROTEIN Russian patent published in 2013 - IPC C12N15/00 

FIELD: biotechnologies.

SUBSTANCE: invention is a vector for production of a vector for expression in a bacterial cell of a precursor of a target recombinant protein, fused with an N-end peptide, containing a decahistidine cluster and a site of recognition with proteinase, substantially containing of a section of initiation of replication of pBR322 ori; a gene of a repressor of a lactose operon; a bacterial promotor; an area coding the N-end leader peptide, containing a decahistidine cluster and, not necessarily, a site of recognition with proteinase; a section of cloning (polylinker); a section of termination of transcription functioning in the bacterial cell; a fragment coding a non-genome pair toxin-antitoxin, at the same time the gene of antitoxin is oriented so that the direction of its transcription matches the direction of transcription of the target gene; the gene coding the bacterial marker of selection. The invention also relates to a vector for expression in a bacterial cell of a precursor of a target recombinant protein fused with an N-end peptide, a bacterium containing such vector and the method for production of the recombinant protein using the bacterium.

EFFECT: invention makes it possible to produce a new vector with high segregation stability for highly efficient expression of recombinant proteins with a leader N-end peptide fused in the frame, containing a decahistidine cluster and a site of recognition with proteinase.

9 cl, 12 dwg, 1 tbl, 4 ex