METHOD FOR PRODUCTION OF APYROGENIC IMMUNOMODULATING AGENT Russian patent published in 2007 - IPC A61K35/60 

FIELD: chemical-and-pharmaceutical industry.

SUBSTANCE: claimed apyrogenic immunomodulating agent represents sodium salt of native low molecular deoxyribonucleic acid. Method for production of said agent includes defatting, grinding and homogenizing of salmon or sturgeon fish roe in citrate-salt aqueous solution at 12000 rpm. Homogenate is centrifuged in settling centrifuge at 3500 rpm under cooling; supernatant is removed; substrate is homogenized again for 3-4 times with addition of calculated amount of citrate-salt solution to form air-and-fat foam followed decantation thereof. Further purified homogenate is incubated in presence of 3-6 % detergent. Saturated sodium chloride solution is added into reaction mass while continuous agitation at elevated temperature. Reaction mass is cooled to 10°C, treated with ultrasound in bath with piezoceramic emitters having vibration frequency of 21.7-23 kHz and moment of resistance at least 9 mum at generator power of 1.5-3 kW. Further liquid phase is separated with sorbent, sorbent is eluted with permeate, eluate, combined with liquid phase is microfiltered, concentrated, sodium deoxyribonucleate is deposited with alcohol, centrifuged, separated from liquid phase, dried under aseptic conditions for 4-6 h at 40-60°C. Amorphous white power containing 95-105.5 % of main substance and nativity above 2.3 is dissolved in 0.9-0.95 % sodium chloride aqueous solution in determined concentrations and poured under sterile conditions in therapeutic dosages at pH 8.0 to produce target immunomodulating agent for intravenous or intramuscular administering.

EFFECT: product of high purity, high nativity and activity.

2 ex, 2 tbl, 2 dwg