METHOD OF MANUFACTURE OF STERILE PHARMACEUTICAL SUBSTANCE Russian patent published in 2018 - IPC A61K35/14 A61K35/20 A61K35/28 A61K35/407 

FIELD: chemistry.

SUBSTANCE: invention relates to the field of chemical and pharmaceutical, food and cosmetic industries and concerns a method for producing substances of preparations of nucleic acids of immunomodulating action. Method for the production of sterile pharmaceutical substance sodium deoxyribonucleic acid from animal raw materials is that the animal tissue is crushed, ballast substances in the form of fat, protein and connective tissue are removed by sequentially separating in the settling centrifuge, first light fractions with a density of up to 0.8 g/cm³ at 2,000 rpm, further medium fractions with a density of up to 1.0 g/cm³ at 3,000 rpm and then the target fraction with a density of more than 1.1 g/cm³ at 4,000 rpm, the target fraction is subjected to lysis, treated with sodium chloride solution, then subjected to ultrasonic treatment, the solution is filtered under vacuum, concentrated by membrane filtration with membrane selectivity from 5,000 to 100,000 Da, the concentrate is treated with ethyl alcohol in a ratio of 1:1–1:2 by volume to form a precipitate of deoxyribonucleic acid sodium salt, the precipitate is dried and subjected to electron beam sterilization or gamma radiation sterilization.

EFFECT: method for the production of sterile pharmaceutical substance sodium deoxyribonucleic acid from animal raw materials is proposed.

3 cl, 2 tbl, 5 ex