FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology and represents method of obtaining L-amino acid, including cultivation of microorganism, belonging to family Enterobacteriaceae, possessing ability to produce L-amino acid and modified in such a way as to increase activity of glycerol dehydrogenase and dihydroxyacetone kinase in medium, containing glycerol as carbon source, for production and accumulation of L-amino acid in medium or in cells, and collecting L-amino acid from medium or cells, with activity of glycerol dehydrogenase and dihydroxyacetone kinase being increased by increasing quantity of copies of genes, coding said fragments, or substitution of prompter of said genes by stronger promoter, and gene of dihydroxyacetone kinase codes protein, selected from group, consisting of: a) protein, containing amino acid sequence SEQ ID NO: 4; and b) protein, containing amino acid sequence, homologous by not less than 95% to amino acid sequence SEQ ID NO: 4, and protein has activity of dihydroxyacetone kinase.
EFFECT: invention makes it possible to obtain at higher level L-amino acids with application of said microorganism, in which dihydroxyacetone kinase uses ATP as phosphate donor.
14 cl, 6 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING L-AMINO ACID | 2009 |
|
RU2518677C2 |
| MUTANT ADENYLATE CYCLASE, DNA CODING IT, BACTERIA OF ENTEROBACTERIACEAE FAMILY CONTAINING SAID DAN AND METHOD FOR PREPARING L-AMINO ACIDS | 2010 |
|
RU2471868C2 |
| MICROBIOLOGICAL METHOD OF OBTAINING 1,2-PROPANEDIOLE | 2009 |
|
RU2521502C2 |
| METHOD OF PRODUCING OF L-LYSINE OR L-THREONINE BY MEANS OF BACTERIA ESCHERICHIA HAVING ATTENUATED ACTIVITY OF MALEIC ENZYME | 2004 |
|
RU2337140C2 |
| METHOD FOR OBTAINING L-AMINO ACID USING BACTERIUM OF Enterobacteriaceae FAMILY, WHICH HAS INCREASED EXPRESSION OF GENES OF STAGE OF FORMATION OF FLAGELLA AND CELLULAR MOVABILITY | 2012 |
|
RU2508404C1 |
| BACTERIUM BELONGING TO ESCHERICHIA GENUS AS PRODUCER OF L-THREONINE AND METHOD FOR PREPARING L-THREONINE | 2004 |
|
RU2288264C2 |
| BACTERIUM PRODUCER OF PRODUCT OF REACTION CATALISED BY PROTEIN SHOWING 2-OXOGLUTARATE-DEPENDENT ENZYME ACTIVITY, AND METHOD FOR PRODUCING SUCH PRODUCT | 2008 |
|
RU2444568C2 |
| NEW MICROORGANISM AND METHOD OF PRODUCING 1,2-PROPANEDIOL BASED ON NADPH-DEPENDENT ACETOL REDUCTASE AND IMPROVED NADPH SUPPLY | 2015 |
|
RU2711870C2 |
| MUTANT PROTEIN, CODED BY GENE yddG, AND METHOD OF OBTAINING AROMATIC L-AMINO ACIDS WITH APPLICATION OF BACTERIA OF GENUS Escherichia | 2012 |
|
RU2530171C2 |
| BACTERIUM BELONGING TO GENUS ESCHERICHIA AS PRODUCER OF L-HISTIDINE AND METHOD FOR PREPARING L-HISTIDINE | 2003 |
|
RU2276687C2 |
