RECOMBINANT PLASMID DNA pE-His8-TrxL-Ar2 ENCODING FUSION PROTEIN COMPRISING ANTIMICROBIAL SEA ANNELID WORM Arenicola marina PEPTIDE ARENICIN AND STRAIN Escherichia coli BL21(DE3)/pE-His8-TrxL-Ar2 AS PRODUCER OF ARENICIN-CONTAINING FUSION PROTEIN Russian patent published in 2008 - IPC C12N15/11 C12N1/21 C12R1/19 

FIELD: biotechnology, genetic and protein engineering.

SUBSTANCE: method involves construction of recombinant plasmid DNA pE-His8-TrxL-Ar2 of size 4059 base pairs encoding fusion protein His8-TrxL-Ar2 comprising antimicrobial peptide arenicin consisting of Bgl II/Xho I DNA fragment comprising transcription promoter T7-RNA-polymerase, ribosome binding site and sequence of fusion polypeptide His8-TrxL-Ar2 comprising histidine octamer, modified thioredoxine A(M37L) and arenicin, and Bgl II/Xho I DNA-fragment of plasmid pET20b(+) comprising transcription terminator T7-RNA-polymerase, replication initiation site and β-lactamase gene determining resistance of Escherichia coli cells transformed with plasmid pE-His8-TrxL-Ar2 to ampicillin as a genetic marker. Also, method involves preparing the strain Escherichia coli BL21(DE3)/pE-His8-TrxL-Ar2 as a producer of fusion protein comprising antimicrobial peptide arenicin. Invention can be used in medicine and pharmaceutical industry.

EFFECT: valuable biological properties of DNA and strain.

3 cl, 2 dwg, 2 ex