FIELD: medicine.
SUBSTANCE: described is a plasmid vector pE-Lc-LTP for protein Lc-LTP expression in Escherichia coli cells, containing a gene of the hybrid polypeptide Trx-Lc-LTP including the amino acid sequence Lc-LTP, a sequence of the protein thioredoxin and an affine mark. Presented is a producer strain of said hybrid polypeptide based on the E.coli bacteria strain. Offered is a method for producing the end protein Lc-LTP by expression of the hybrid polypeptide Trx-Lc-LTP containing it in the cells of said strain, cell destruction, affine purification of the hybrid polypeptide Trx-Lc-LTP on a metalchelate carrier, hybrid protein breakdown in a site located between the sequences Lc-LTP and thioredoxin, and purification of the end protein Lc-LTP by reverse phase HPLC. The invention can find application both in agriculture as factors improving plant resistance to infections, and in medical practice for food allergy diagnostics and treatment.
EFFECT: enhanced efficiency and simplification of the technology of lipid-transporting lentil proteins Lens culinaris.
9 cl, 4 dwg, 6 ex
