FIELD: biotechnology, peptides.
SUBSTANCE: invention relates to a method for preparing peptide arenicin possessing the antimicrobial activity. Method involves culturing the recombinant strain-producer Escherichia coli BL21(DE3)/pE-His8-TrxL-Ar2 in nutrient medium followed by disruption of bacterial cells, isolation of inclusion bodies comprising fusion protein His8-TrxL-Ar2. Then this fusion protein is purified by the method of metal-chelating chromatography, subjected for solubilization and the following splitting with cyanogen bromide by methionine residue in acid medium. Then reaction products are separated and arenicin is subjected for final purification by reversed-phase HPLC method. Using the invention provides expanding assortment of antimicrobial preparations of broad spectrum of effect and to carry out the high-technological effectiveness of production of arenicin. Prepared peptide is similar to peptide isolated from annelid worm Arenicola marina and can be used in medicinal and veterinary practice as antibiotic of the broad spectrum of effect.
EFFECT: improved preparing method of peptide.
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