FIELD: biotechnologies.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of proteins, and can be used for production of Antimicrobial Peptide acipensine-1 of Russian sturgeon Acipenser gueldenstaedtii. Constructed plasmid vector pET-His8-TrxL-Acip1 acipensine-1 expression in Escherichia coli cells as a part of hybrid protein His8-TrxL-Acip1. Transformed data vector of parental Escherichia coli BL21 (DE3) and strain-producer of hybrid protein His8-TrxL-Acip1. That is followed by culturing the strain producer cell lysis is performed, affine purification of hybrid protein His8-TrxL-Acip1 on metalchelate carrier, hybrid protein splitting His8-TrxL-Acip1 cyan at residue of methionine, inserted between the sequences acipensine-1 and thioredoxin, repeated performing cleaning and final purification of the target peptide by reverse-phase HPLC.
EFFECT: invention allows to produce a biologically active acipensine-1 using a simple technique.
3 cl, 4 dwg, 4 ex
Title |
Year |
Author |
Number |
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