PLASMID VECTOR pET-His8-TrxL-Acip1 STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET-His8-TrxL-Acip1 FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE ACIPENSINA-1 AND METHOD OF PRODUCING SAID PEPTIDE Russian patent published in 2016 - IPC C12N15/12 C12N15/62 C12N15/70 C12P21/02 C12N1/21 C12R1/19 

FIELD: biotechnologies.

SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of proteins, and can be used for production of Antimicrobial Peptide acipensine-1 of Russian sturgeon Acipenser gueldenstaedtii. Constructed plasmid vector pET-His8-TrxL-Acip1 acipensine-1 expression in Escherichia coli cells as a part of hybrid protein His8-TrxL-Acip1. Transformed data vector of parental Escherichia coli BL21 (DE3) and strain-producer of hybrid protein His8-TrxL-Acip1. That is followed by culturing the strain producer cell lysis is performed, affine purification of hybrid protein His8-TrxL-Acip1 on metalchelate carrier, hybrid protein splitting His8-TrxL-Acip1 cyan at residue of methionine, inserted between the sequences acipensine-1 and thioredoxin, repeated performing cleaning and final purification of the target peptide by reverse-phase HPLC.

EFFECT: invention allows to produce a biologically active acipensine-1 using a simple technique.

3 cl, 4 dwg, 4 ex