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2 317 545

(13)

(51)

IPC

G01N33/487(2006-01-01)

(21) (22)

Application

2006118092/15, 2006-05-25

(24)

Start date

2006-05-25

(22)

Actual filing date

2006-05-25

(45)

Published

2008-02-20

(72)

Inventor

Gazdalieva Luiza MavletovnaKulagina Irina Gennad'EvnaKamilov Feliks Khusainovich

(73)

Holder

Gosudarstvennoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Gosudarstvennyj Meditsinskij Universitet Federal'Nogo Agentstva Po Zdravookhraneniju I Sotsial'Nomu Razvitiju" Vpo Bgmu Roszdrava)

METHOD FOR DETERMINATION OF NITRATE AND NITRITE IN BIOLOGICAL MEDIUM OF SMALL VOLUME Russian patent published in 2008 - IPC G01N33/487

Abstract RU 2317545 C1

FIELD: medicine, clinical biochemistry.

SUBSTANCE: invention relates to a method for determination of nitrates and nitrites in lacrimal fluid. Method involves precipitation of proteins with 1.5 ml of 96% ethanol cooled to +4-8°C and 0.1 ml of distilled water in the final concentration 86.4% and then 2 ml of ammonium-chloride buffer at pH 9.6 ± 0.05 is added and volume is brought about to 10 ml. Then nitrates and nitrites are isolated through a cadmium column, color reaction is carried out with addition of N-naphthylethylenediamine dihydrochloride followed by photometry and determination of concentration of nitrates and nitrites by plot showing dependence of concentration from optical density. The concentration of nitrates and nitrites are calculated by the following formula: NO_x (mcmole/l) = wherein C₁ means concentration of nitrite ions calculated by calibration plot; NO_x =

NO^- ₂/NO^- ₃. Invention allows assay of concentration of nitrates and nitrites in biological media of small volumes.

EFFECT: improved method of determination.

1 dwg, 2 ex

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RU 2 317 545 C1

Authors

Gazdalieva Luiza Mavletovna

Kulagina Irina Gennad'Evna

Kamilov Feliks Khusainovich

Dates

2008-02-20—Published

2006-05-25—Filed