METHOD FOR EVALUATING THE INTENSITY OF INFLAMMATORY REACTION IN SURGICAL WOUND Russian patent published in 2008 - IPC G01N33/48 C12Q1/02 

FIELD: medicine, surgery.

SUBSTANCE: the present innovation deals with evaluating the intensity of inflammatory reaction in surgical wound due to studying bactericidal activity of wound secreta. One should take 20 mcl recombinant test-strain E.coli that carries genes of lux-operon of marine luminescent bacterium Photobacterium leiognathi, at concentration ranged 10⁷-10⁸ CFU/ml, mix with equal volume of wound secreta (experiment) and 0.85%-sodium chloride solution (control). The both samples should be kept at 37°C for 20 min. One should apply upon a slide and calculate the quantity of luminescent cells/area unit. It is necessary to calculate the coefficient of bactericidity (Cb) by the following formula: where Ac and Ao - average value of the quantity of luminescent objects detected at automatized calculation of 10 vision fields in control (c) and experiment (e), correspondingly. At Cb>60% the intensity of inflammatory reaction should be evaluated to be high, that corresponds to the development of infectious-inflammatory complication in the area of surgical interference. The innovation enables to apply the method in case of any diseases that require surgical therapy, moreover, invasive procedures are excluded, the specificity of the method has been increased.

EFFECT: higher efficiency of evaluation.

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